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文献和实验ES CELL DNA EXTRACTION: TUBE ME
in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM Tris-HCl pH7.5 - 0.5 ml of 2M 10 mM EDTA - 2 ml of 0.5M 10 mM NaCl - 0.2 ml
ES CELL DNA EXTRACTION: TUBE ME
Protocol for extracting DNA from ES Cells, starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM
Dynamic Flow Assay in a Parallel Plate Flow Chamber
media serum-free with 12 mM Hepes) Fibronectin (human plasma) Bovine serum albumin (BSA) Flow Chamber System: Flow chamber deck with gasket and tube fittings 35 mm tissue culture
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