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文献和实验Co. 48500-00 Soft Tubing Cole Parmer Instrument Co. 96400-16 Syringe filter
- 140 Volts) for 40 hrs until the XC reaches about 33 cm from the top (BPB indicates about 65 bp and XC indicates about 260 bp). I. Gel drying Cover the gel with a piece of filter paper (3 MM, 345 mm x 425 mm). Cut off the gel
7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
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