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文献和实验tubes for use in the machine 5.Ultrafree-MC(R) filter units, 0.45 μm Procedures: 1.Quick thaw the gel piece in a 600-μl tube using the PCR machine at 37 ℃ 2.Macerate the gel piece with a spatula. 3.Transfer into the sample cup of the filter unit
2.Frozen agarose gel piece containing the desired DNA fragment Supplies: 1.Micropipetter and tips 2.Microcentrifuge and tubes 3.Spatula 4.PCR machine and the 600-ul tubes for use in the machine 5.Ultrafree-MC
onto the nitrocellulose d. Place 8 more soaked pieces of filter paper on the gel. e. Purge the stack of any air bubbles with a glass pipette. f. Assemble the cathode plate and start the transfer 7. The equation to calculate the amperage
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