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文献和实验DNeasy 96植物技术:使用搅拌磨MM 300从新鲜植物叶片中分离DNA
between adapter plates and fix into the mixer mill clamps as described in the Mixer Mill MM 300 instruction manual (如《拌磨MM 300 使用手册》描述的那样,将每个微型收集管架插入到两个适配微孔板中,并用搅拌磨夹加以固定)Note: Ensure that the microtubes are properly sealed with caps(注意:确保收集微型管盖封好)。IMPORTANT
Native Chromatin Preparation and Illumina/Solexa Library Construction
1. On the day of the experiment, obtain a leukapheresis pack from a blood bank. Dilute 109 leukocytes with 1X PBS (1:1). Pipette 15 mL of LSM into a 50-mL conical tube. Carefully layer the lymphocyte suspension over the LSM. A clear boundary
The ribonuclease protection assay (RPA)
by gentle flicking and quick spin in a microfuge. Incubate at 37°C for 30 minutes. 3. Add the following reagents (in order) to each 1.5 ml Eppendorf tube: 26 µl 20 mM EDTA 25 µl Tris-saturated phenol 25 µl chloroform:isoamyl alcohol (50
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