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文献和实验Endothelial Cell Systems - Instructions For Use
, cell viability, and seeding density. USE ONLY CLONETICS® TRYPSIN/EDTA WHICH HAS A CONCENTRATION OF 0.025% TRYPSIN/O.O1% EDTA. THE CONCENTRATION OF TRYPSIN/EDTA FROM OTHER SUPPLIERS MAY BE AS HIGH AS 0.25% TRYPSIN (=10x THE RECOMMENDED
Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别
can be solved in 60o C water bath. The final concentration of phenol is 38% x 1.2 = 45.6%. Add water to 200 ml total volume. pH ~ 4.5. Wrap with aluminum foil. RNA precipitation solution: 200 ml NaCl 1.2 M 14.026 g FW 58.44 Sodium Citrate 0.8M 47.056
Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别提取细胞核和细胞质RNA
T℃OS-M6 cells grow in 6-well plates, 2ml media totalT℃V1PD cells grow in 100mm plates.1: Aspirate off the media from the cells and was the cell monolayers with 2x5ml (To 100mm dish) or 2x1ml (to 6-well plates) ice cold TD (4o C is OK
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