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文献和实验Field-Inversion Gel Electrophoresis
box and a power supply is a device enabling the periodic inversion of the electric field direction over the course of the experiment. This method was derived from experiments done on a modified orthogonal-field-alternation gel electrophoresis (OFAGE
SEMI-DRY ELECTROPHORETlC TRANSFER (WESTERN BLOTS)
that it is just as effective to use a slightly diluted version of the Tris/glycine running buffer used for SDS-polyacrylamide gels. Materials (for one transfer) Power supply capable of 12 - 100 V, 5 W 1 - 7 x 10 cm nitrocellulose sheet, 0.45 or 0.22 um pore
Marcantonio Lab Protocol Manual
with 500 ml of TBE electrophoresis buffer in each. 6) Close the upper and lower safety lids and attatch the apparatus to the power supply. (Red lead on bottom, black lead on top) Gel should be pre-electrophoresed at 30 watts, constant power, for 15-30
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