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文献和实验especially if the BioBrick is less than 100 bp since in my experience short fragments don''t fuse together as well as larger fragments. Primers for Part A (upstream part = insert) + Part B with the pSB1A2 vector (downstream part = vector):
A Guide to Yeast Two-Hybrid Experiments（酵母双杂交实验指南）
is carried out inside the nucleus of the yeast Saccharomyces cerevisiae, where the two proteins to be tested are expressed as hybrid molecules (Figure 1A). One is fused to the DNA-binding domain (BD) of a transcription factor such as Gal4 or LexA
Microtubule-associated protein 1A/1B-light chain 3 (LC3) is a soluble protein with a molecular mass of ∼17 kDa that is distributed ubiquitously in mammalian tissues and cultured cells. During autophagy, autophagosomes engulf cytoplasmic
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