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文献和实验Medium Per Liter: To 950 ml of deionized H2 O , add: Tryptone 20 g Yeast Extract 5 g NaCl 0.5 g Shake until the solutes have dissolved. Add 10 ml of a 250 mM solution of KCl. (This solution is made by dissolving
Materials Bunsen burner Wire loop Petri plate or broth tube Bacterial culture Microscope slides Procedure 1. Pick up the inoculating loop and hold it pointed down into an open flame until the loop glows red
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
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