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文献和实验Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
, Wheaton). Homogenize using a loose pestle with 25 strokes. Transfer to 2 ml Eppendorf tubes and pellet the nuclei by centrifugation at 1500 g for 5 min. Transfer the supernatant to a new tube and clear it by spinning 15 min in a tabletop
). II. Procedure A. Cell treatment and collection Farm L929 cells on 150mm plates (10 plates yeilds approx. 550ug of protien). Treat cells with latex beads (Polybead polystyrene microspheres 4.55 x 10 beads/ml
Medium Per Liter: To 950 ml of deionized H2 O , add: Tryptone 20 g Yeast Extract 5 g NaCl 0.5 g Shake until the solutes have dissolved. Add 10 ml of a 250 mM solution of KCl. (This solution is made by dissolving
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