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文献和实验as needed. Ex. To make 100 ml of TE buffer (10 mM Tris, 1 mM EDTA), combine 1 ml of a 1 M Tris solution and 0.2 ml of 0.5 M EDTA and 98.8 ml sterile water. The following is useful for calculating amounts of stock solution needed: Ci x Vi = Cf
μM calyculin A, 2 mM PMSF, and 10 μg/ml aprotinin). 4) Lyse for 20 min on ice and clarify lysates by centrifugation at 16,000 x g for 10 min at 4 oC 5) Preclear lysates with 30 μl of a 1:1 slurry of protein G-sepharose (Fast-Flow; Pharmacia
Endothelial Cell Systems - Instructions For Use
: cells/ml = (n) x 104, where: n = the average cell count per square of the four corner squares counted. Example: If the calculated average (n) of cells in the four 1 mm corner squares of the hemacytometer is 30: cells/ml = (n) x 104 (or) cells/ml
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