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文献和实验Replication timing by density transfer
(frozen slanted to maximize the surface area). During the experiment, mix 20 ml of cells with 1/50 volume of 10% Na-azide in a 35 ml Corex tube (on ice) and immediately transfer the mix to a tube of frozen EDTA/azide. Alternatively, squirt the 10% azide
Replication timing by density transfer
(frozen slanted to maximize the surface area). During the experiment, mix 20 ml of cells with 1/50 volume of 10% Na-azide in a 35 ml Corex tube (on ice) and immediately transfer the mix to a tube of frozen EDTA/azide. Alternatively, squirt the 10% azide
membrane in transfer buffer. Assembly of the Unit for Standard Transfers (Wear gloves for this procedure to avoid contamination of membranes.) 1. Remove the safety cover and the stainless steel cathode assembly. 2. Place
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