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文献和实验dryer (Life Technologies)Glass plates, 31.0 x 38.5 cm (Life Technologies)0.4 mm spacers (Life Technologies)Casting boot (Life Technologies)Shark tooth comb (Life Technologies)Small clampsWhatman blotting paper, 3 mm thicknessKodak Biomax MR or AR
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
. Most amoebae are negatively phototropic, that is, they shy away from light. As the amoeba moves it will shoot out pseudopodia that are well attached to a glass surface (the surface could be the slide or the underside of the cover slip). Pseudopodia
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
(Vector Laboratories PK 6100) (optional, see Step 30) Wash solution 1 Wash solution 2 Equipment Aluminum foil Diamond pen Eye protection (see Step 21) Filter paper (e.g., Whatman 3MM) Humid chamber for slide
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