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文献和实验GST fusion protein purification GST融合蛋白纯化
resuspending on ice circa 10'after thawing.3)transfer to 50ml conical ss34 flip top tubes.4)add 10mg lysozyme powder to the 50ml (cells from 1 liter now in 1 50ml tube),digest 15' on ice.5)sonicate with large probe 1' 80% power,freeze in lN,thaw at37℃
Southern Hybridization Protocols
8.0 20X SSC 50X Denhardts 20% SDS *SS DNA 10 mg/ml16.00 ml 1.25 ml 0.50 ml 6.25 ml 0.50 ml 0.25 ml 0.25 ml 25.00 ml- 50 mM Tris 10mM EDTA 5 x SSC 1 x Denhardts 0.2 SDS 100 m g/ml* Boiled for 10 min then cooled on ice. Then added to the solution at 65
GST fusion protein purification--GST融合蛋白纯化
ml tube),digest 15' on ice.5)sonicate with large probe 1' 80% power,freeze in lN,thaw at37℃ sonicateagain on ice,solution should become viscous.6)add 1mg DNAse and RNAse,incubate on ice 15'.7)spin 7.5k rpm4℃ss34 10'.8)transfer supernatants to conical screw
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