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文献和实验ensures a low background level of nonspecific probe adherence to the membrane and, second, an extended period of filter prehybridization is not required. The inclusion of a large amount of SDS does, however, necessitate that the nucleic acids
binds to a specific DNA sequence, passage through the filter will result in retention of a fraction of the protein-DNA complex by virtue of the protein component of the complex. The amount of DNA retained can be determined by using radioactively labeled
Microwave Processing Techniques for Biological Samples in a Service Laboratory
The author’s interest in microwave (MW) processing of biological samples for electron microscopy was initially stimulated by presentations and papers by Dr. Gary Login (Login and Dvorak, 1988 , Login and Dvorak, 1993 , Login and Dvorak, 1994
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