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- Beckman Coulter贝克曼库尔特
- 美国
- C63401
- 2025年07月10日
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文献和实验by inverting the bottle a few times, and incubate in an ice-water bath for 30-60 minutes. 6. Clear the lysate of precipitated SDS, proteins, membranes, and chromosomal DNA by pouring through a double-layer of cheesecloth. Transfer the lysate into 250 ml
Microarray Hybridization Protocol
on it. Therefore, the first thing to do is to wash them.) Place bottle of Microhyb solution (Research Genetics HYB250.GF) at 65° C at least 15 minutes before use. Denature 1 ml of tRNA (50 ?g/?l) (Sigma R-8759) at 95 ?C for 10 minutes.
Genomic (Plant) DNA: Restriction Digestion for Sou
towels. Set a 500g weight (e.g., an Erlenmeyer flask with 500 ml H2O) on top of the flat support. Go home. Disassembling the Blot: Disassemble the blot. Discard the Saran, the Gel Blot Paper and any paper towels that are wet. Remove
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