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文献和实验debris. Transfer the supernatant to a fresh tube. 17x100mm disposable polypropylene tubes (Falcon brand has pop-top cap) are convenient. These can be rinsed and re-used. 6.Add DNase I (final is 1μg/ml) and RNase AI (final is 10μg/ml). For stocks
DNAExtractionProtocolsUsingSilica
. Höss recommends 500 µL aliquots wrapped in aluminum foil (stable for 6 months at room temperature). B. Extraction buffer 1. Combine 24 g GuSCN (guanidinium thiocyanate) and 20 mL 0.1 M Tris-HCl (pH 6.4) in a 50 mL Falcon tube. 2. Heat to 60 oC
DNA Extraction Protocols Using Silica
Below we present the protocols we have used to isolate DNA from various tissues using Silica and Guanidinium Thiocyanate.These protocols are adapted from Boom et al. (1990),Höss & Pääbo (1993),and Höss (1994).We highly recommend reading
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