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文献和实验Single tube confirmation PCR protocol
The following colony PCR protocol has been designed to be performed in individual reaction tubes. We usually test three colonies from each transformation along with a wild-type control. A series of five different PCR
. Worms dyed late in the afternoon can be stored at 15°C overnight (o/n) and viewed the next morning. Microfuge tube Label 1.7 ml microfuge tube with strain name. Squirt ~1 ml M9 onto plate with a pasteur pipet. Rinse plate with M9
Single tube confirmation PCR protoco
µl of the PCR mix to each of the 20 PCR tubes that already contain the 15 µl of primers and template. 110 µl 5 µl 10 x Taq buffer(see below) 11 µl 0.5 µl 20 mM dNTP's (0.2 mM) 11 µl 0.5 µl Taq Polymerase
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