Kit, TUBE CAP AY, AL 1/2" DIA

Targeted Differential Display

of the sample RNA: A positive control prepared with known, high-quality RNA A negative control prepared from either a LCM cap containing no tissue or RNase-free water Prepare sufficient volume of the following reaction mixture for each tube:   Add

Whole genome amplification protocol for ChIP-chip

to a genomic tiling array. Therefore, we have adapted the standard protocol for whole genome amplification using the Sigma GenomePlex WGA kit to amplify our ChIP sample (O'Geen et al., 2006). Using Oct4 ChIP-chip assays as an example, we have compared

Plant DNA methylation analysis by bisulfite genomic sequencing

and Wassenegger (2000). Back to top Procedure Preparation of Plant Genomic DNA Prepare plant genomic DNA using a Qiagen DNeasy Plant Maxi or Mini kit (see comment 1). Prior to the bisulfite-treatment, the plant genomic DNA is cleaved