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- 2025年07月13日
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文献和实验Purification of Plasmid from 50 ml-culture
the supernatant. Remove all of the fluid using pipetman. 10. Dissolve the pellet in 600 ul of TE. Transfer the solution into a microfuge tube. 11. Add 200 ul of 8M LiCl. Mix well, and then spin the solution at 14,000 rpm for 5 min at 4 C. 12
ES CELL DNA EXTRACTION: TUBE ME
in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM Tris-HCl pH7.5 - 0.5 ml of 2M 10 mM EDTA - 2 ml of 0.5M 10 mM NaCl - 0.2 ml
He Tube Mating with MSCV recipient
. 3. Set up Dilutions: Set up dilutions as shown below, in 5 ml LB+0.2% rhamnose. Use 50 ml Falcons for this as they have good aeration and you get much better growth. Place these dilutions at 30℃ shaking for 2 hours. pMSCV: 25 μl 5ml 50 μl 5ml
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