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文献和实验All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
Brief protocol using Digest-All™ Kit is described below. The Digest-All™ is a flexible and standardized system for tissue digestion and contains trypsin, ficin, and pepsin proteolytic enzymes. The degree of digestion is based on a standardized 10 minute
【转贴】immunofluorescence staining
Kit (Molecular Probes), coverslips can (optionally) be incubated with 1-2 drops of Equilibration Buffer for 5-10 min. before mounting.Place a drop of mounting media on the slide for each coverslip to be mounted (two drops for square coverslips
Native Chromatin Preparation and Illumina/Solexa Library Construction
Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies
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