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文献和实验In Vitro Models of the BloodCerebrospinal Fluid Barrier and Their Use in Neurotoxicological Research
The choroid plexus epithelium forms the interface between the blood and the cerebrospinal fluid. In addition to its barrier function resulting from the presence of tight junctions sealing the epithelial cells
【转贴】immunofluorescence staining
off the parafilm. Sealing the edge of the dish with tape or parafilm during incubation, plus placing a small wad of wetted kim-wipe in the dish, will keep the coverslips from drying out. Note: This protocol has been optimized for HeLa cells, and works
Yale University Policy for Aseptic Amphibian Survival Surgery
by several methods: Steam : 250°F., 15 p.s.i., for 30 minutes. Gas-Ethylene oxide: Exposure requires 8-10 hours in a special cabinet (gas autoclave). Plastic implantable materials must be aerated for two to seven days, depending on equipment
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