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文献和实验Fluorochrome Absorption and Emission Spectra
daltons and an absorption maximum at 495 nm (Fig. 2). Its excitation by 488-nm light leads to a fluorescence emission maximum around 520 nm (Fig. 2). Using a 530 ?15 nm bandpass (BP) filter will give optimum detection for this fluorochrome
Fluorochrome Absorption and Emission
daltons and an absorption maximum at 495 nm (Fig. 2). Its excitation by 488-nm light leads to a fluorescence emission maximum around 520 nm (Fig. 2). Using a 530 ?15 nm bandpass (BP) filter will give optimum detection for this fluorochrome
for eGFP, 610 nm longpass filter for PI, 675/20 nm bandpass filter for Cy5 and a 424/44 nm bandpass filter for DAPI. Doublets were excluded by pulse processing. Cells expressing eGFP were gated and analysed for either Cy5- or PI-fluorescence. Data
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