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文献和实验Myosin Light Chain Preparation
the 8 liter and the 28 liter beakers. 5.Put ETOH in the cold room. 6.Refrigerate or equilibrate the DE52 to be used. 7.Find parts to and assemble the 5½ cm ID column. 8.Make sure there is plenty of cold water in the cold room. 9.Thaw 8 M urea. Solutions
Chick Chorioallantoic Membrane (CAM) Assay
for gross evaluation. For injection, borosilicate glass capillaries (OD 1.0 mm, ID 0.75 mm; Sutter Instrument Company, Novato, CA) were prepared with P-87 micropipette puller (Sutter Instrument Company). Needles were connected with Tygon tubing (ID 1/32
not to disturb the phosphocellulose resin. As the adjuster plunger is inserted into the column, the column inlet tubing that is attached to the plunger should be immersed in a 4L reservoir of column buffer, and should expel all air bubbles as the adjuster
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