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- VWRV1B1493
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
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见瓶身
- 保质期：
/
- 英文名：
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999
- 供应商：
麦飞生物
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1 L
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文献和实验with a strain that already has one or more plasmids use liquid drop-out media lacking the appropriate nutrient.b Incubate at 30o C in roller drum at top speed overnight. b 2. Measure the OD600 of the overnight culture.c Make an appropriate dilution
Two-hybrid analysis of genetic regulatory networks
and inoculate 0.5 ml of freezing solution in a cryotube. Vortex lightly. This solution should have an OD600 over 3.0. 3. Alternatively, inoculate 0.5 ml of -u-h Glu liquid media to an OD600 less than 0.2, incubate at 30oC with shaking until OD
penicillin (base)/mL and 10,000 μg streptomycin(base)/mL in 0.85% NaCl (liquid) (see Note 2 ). 3. Dulbecco’s phosphate-buffered saline (PBS). 4. Trypsin (0.25% liquid trypsin). 5. Cell culture dishes: 10- or 15-cm dishes or large surface areaculture
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