货号：VWRV0167 AMRESCO GLYCINE

免疫沉淀-蛋白印迹法

x stock Tris/ Glycine SDS buffer were from National Diagnostics, (Hull, UK).eNOS (NOS 3) affinity purified rabbit polyclonal antibody was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, Calafornia, USA).Goat Anti-Rabbit horseradish peroxidae

顽拗性植物组织的蛋白质组学研究（苯酚法提取蛋白质）

多糖蛋白。 苯酚提取法有高度的清洁能力。它能够作为一种分离剂，降低蛋白质和其他分子间的相互影响。苯酚提取法最主要的不足在于它时间消耗过长（至少 6 h) ，另外苯酚和甲醇都是有毒的。2. 材料 2.1 苯酚 饱和 Tris-HCl 缓冲液，pH 6. 6/7.9  ( Amresco- Interchim， biotechnology  grade) 。 2.2 提取缓冲液 准备一份含有 500 mmol/L Tris-HCl 缓冲液、500 mmol/L EDTA、700 mmol

RNA-chromatin immunoprecipitations (RNA-ChIP) in mammalian cells

minutes at room temperature (see note 1). Glycine was added to a final concentration of 125mM to quench crosslinking, and the cells pelleted again. The pellet was washed twice with ice-cold PBS containing 1x protease inhibitor cocktail.