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文献和实验freeshotgun sequencing library from miniprep BAC DNA
Pulse Field Gel Electrophoresis Apparatus. Load New England BioLabs Lambda Ladder PFG Marker ( #340) on the gel. Stain with ethidium bromind and evaluate. 8.Isolate inset DNA Isolate DNA from agarose gel slice(s) depending on the insert
free shotgun sequencing library from miniprep BAC DNA
Lambda Ladder PFG Marker ( #340) on the gel. Stain with ethidium bromind and evaluate. 8.Isolate inset DNA Isolate DNA from agarose gel slice(s) depending on the insert size and with/without Not 1 cut site in the insert of individual BAC clone
and 0.25% (w/v) Bromophenol Blue) 100 bp Size Standards DNA ladder (1 mg/ml) 50 µl 1 M Tris-HCl (pH 8.0) 5 µl 0.5 M EDTA (pH 8.0) 5 µl Loading Buffer 440 µl 500 µl Method 1. Cast a 2% agarose gel (1X TAE) with four combs (50
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