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- 文献和实验
- 技术资料
- 保存条件：
见瓶身
- 保质期：
/
- 英文名：
/
- 库存：
999
- 供应商：
麦飞生物
- CAS号：
50-99-7
- 规格：
100 ml
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文献和实验Replication timing by density transfer
C]- acetate or glucose 0.01% (w/v) [15 N]- ammonium sulfate Amino acids/supplements (as required, added to the same concentration as in normal synthetic medium) --dissolved in "-N" medium and filtered
The Inoue Method for Preparation and Transformation of Competent E. coli: "Ultra Competent" Cells Joseph Sambrook Peter Maccallum Cancer Institute and The University of Melbourne
Transient transfection into 293T cel
. They (both) have the unusual property of being highly transfectable by the following Ca3 (PO4 )2 transfection protocol. Up to 50% efficiency is attainable.Materials• Complete medium: DMEM(high glucose) supplemented w/ 10% FCS (either w/ or w/o heat inactivation at 55°C/30min
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