货号：VWRV1B1491 AMRESCO RIBORE

差示反转录PCR[mRNA差异显示技术]

PTC-100基因扩增仪（Techne公司，英国），GeneGenius 凝胶成像系统（SYNGENE公司，美国）。-70℃超低温冰箱(Thermo Forma，美国),PCR超净台(基因有限公司，美国),DYY-Ⅲ-5型稳压稳流电泳仪(北京六一仪器厂), 5810R低温冷冻离心机(Eppendorf，德国),Micro-MB3616型高速离心机(IEC公司，美国),Vortex GENIE2(基因有限公司，美国),GeneQuant核酸定量仪(Pharmacia Biotech 公司,英国)。CS

生物芯片实验手册（NIH）

are then purified and spotted onto poly-L-lysine coated microscope slides. In the second operation, BASIC PROTOCOL 2, RNA EXTRACTION AND LABELING, RNA is extracted from the cell samples to be examined, purified, and used as the substrate for reverse transcription

Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells

  INTRODUCTION The mechanism of RNA interference has emerged as a practical tool to study loss of function in many organisms. To make the method of gene knockdown suitable for mammalian cells, short