234400-5ML-M SIGMA Complemen

Measurement of Complement Hemolytic Activity, Generation of Complement-Depleted Sera, and Production of Hemolytic Intermediates

All of the basic functional assays of complement activity utilize erythrocytes as targets (1 ). For classical pathway assays, the favored target is the antibody-sensitized sheep erythrocyte. For alternative pathway assays, unsensitized rabbit

ELISA Procedure for Measuring Serum Antibody Titer

. 2. Remove the unbound conjugate by washing with PBS-T. 3. Add 200 µL of a 10 mg/mL so, lution of p-nitrophenyl phosphate in 0.05 M sodium carbonate buffer (pH 9.8, containing 1 mM MgCl2), slowly at room temperature until a yellow color develops

Immunoaffinity Methods for Purification of Complement Components and Regulators

. Example protocols will be provided for a serum complement protein and a membrane bound complement regulator. Table 1  Modifications of Basic Protocol for Specific Components Componen