Cyanine5.5 抗 H2A.X-磷酸化 (Ser139

Cyanine5.5 anti-H2A.X-Phosphorylated (Ser139) Antibody/PerCP详细如下：

Description ：H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein issynthesized in the G1 and S phase of the cell cycle and is known to be important for DNArepair and maintaining genomic stability and for recombination between immunoglobulin switchregions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks.Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shownto be useful for Western blotting, immunofluorescence and flow cytometry.

Verified Reactivity ：Human, Mouse

Antibody Type ：Monoclonal

Host Species ：Mouse

Immunogen ：Modified peptide

Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation ：The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5under optimal conditions.

Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)

Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.

Application ：ICFC - Quality tested

Recommended Usage ：Each lot of this antibody is quality control tested by intracellular immunofluorescent staining withflow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl permillion cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibody for FlowCytometry：

1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.

2. Prepare cells of interest.

3. Wash 1Xwith PBS, centrifuge at 350g for 5 min.

4. Discard the supernatant and vortex to loosen cell pellet.

5. Add pre-cooled 70% ethanol drop by drop, while vortexing.

6. Incubate at -20°C for 60 minutes.

7. Wash 3Xwith BioLegend Cell Staining Buffer and resuspend the cells at 0.5-1 X 10 cells/ml inthe cell staining buffer.

8. Perform immunofluorescent staining for flow cytometry.


Application References(PubMed link indicatesBioLegend citation)：

1. Jha JC, et al. 2013. J. Virol. 87:5255. (FC) PubMed

2. Akbay A, et al. 2008. Am J Pathol. 173:536. (IHC) PubMed

3. Mochizuki K, et al.2008.J cell Sci.121:2148. (IF) PubMed

4. Xiao R, et al. 2007. Mol Cell Biol.27:5393. (IF) PubMed

5. Rossi DJ, et al. 2007. Nature. 447:725. (IF) PubMed

6. Loidl J, et al. 2009. Mol Cell Biol. 20:2048. (IF) PubMed

7. Beels L, et al. 2009. Circulation. 120:1903. (IF) PubMed

8. Suzuki K, et al. 2010. Nucleic Acids Res. 38:e129. (IF) PubMed

9. Lukaszewicz A. 2010. Chromasoma Apr 27. [Epub ahead of print] (IF) PubMed

10. Yamada C, et al. 2010 J. Biol. Chem. 285:16693. (WB) PubMed

11. Bu Y, et al. 2010, Biochem Biophys Res Commun. 397:157. (WB) PubMed

12. Massignan T, et al. 2010. J. Biol Chem. 285:7752. (WB) PubMed

13. Banath JP, et al. 2010. BMC Cancer 10:4 (FC)

14. Zhang M., et al. 2011. Cancer Res. 23:7155. PubMed

15. Kuefner MA, et al. 2012. Radiology 264:59. PubMed

16. Yoshihara Y, et al. 2012. Biochem Biophys Res Cmmun. 421:57. PubMed

17. Titus S, et al. 2013. Sci Transl Med. 13:21. PubMed

18. Crown KN, et al. 2013. G3. 6:1927. PubMed

19. Schenkwein D, et al. 2013. Nucleic Acids Res. 41:e61. PubMed

20. Zhadanova NS, et al. 2014. Mol Cell Biol. 34:2786. PubMed

21. Horrell SA, et al. 2014. Eukaryot Cell. 13:1300. PubMed

22. Maya-Mendoza A, et al. 2015. Mol Oncol. 9:601. PubMed

Product Citations：

1. Carpenter RS, et al. 2020. Nat Commun. 3.029166667. PubMed