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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
868
- 靶点:
CD40
- 级别:
CD40 Antibody
- 目录编号:
CD40 Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
CD40 Antibody
- 抗体英文名:
Cyanine7 anti-mouse CD40 Antibody-PE
- 抗体名:
CD40 Antibody
- 标记物:
PE
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
CD40 Antibody
- 亚型:
CD40 Antibody
- 形态:
CD40 Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/ml
- 规格:
25 µg/100 µg
| 规格: | 25 µg | 产品价格: | ¥1900.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥5900.0 |
Description :CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of thetumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelialcells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switchingand, in combination with other signals such as IL-4, protects B cells from surface Ig-inducedapoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), whichis expressed on activated T cells, is important in costimulation and immune regulation.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :Recombinant mouse CD40 protein
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 underoptimal conditions.
Concentration :0.2 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Hasbold J, et al. 1994. Eur. J. Immunol. 24:1835.
2. Bourgeois C, et al. 2002. Science 297:2060.
Product Citations:
1. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
2. Pierini S, et al. 2021. J Immunother Cancer. 9:. PubMed
3. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
4. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
5. Ciecko AE, et al. 2019. Cell Rep. 29:3073. PubMed
6. Wu G, et al. 2020. Br J Pharmacol. 177:5063. PubMed
7. Marangoni F, et al. 2021. Cell. . PubMed
8. Huang YL, et al. 2015. Nat Commun. 6: 8141. PubMed
9. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
10. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
11. Fontinha D, et al. 2015. PLoS One. 10: 0142540. PubMed
12. Zhang J, et al. 2018. Sci Rep. 8:2373. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
出现IgG 分子50 and 25 kDa 的重链和轻链条带。 5 二抗的选择 二抗应选用与使用的一抗相同的物种来源,例如:如果你的一抗是小鼠的单克隆抗体,二抗则选抗小鼠的二抗anti-mouse secondary。建议检查二抗说明书确保该抗体适用于你的检测应用, 二抗一般连接荧光素FITC 或发光团。 6 双重染色抗体的选择用未偶联一抗进行细胞培养物或组织切片的双重免疫染色要求一抗来源于不同物种并且二抗分别识别其中之一,二抗说明书应描述其与其它物种来源的免疫球蛋有否有交叉吸附。 想了解
69、CD38 等活化指标,及 IFN-γ、IL-4、IL17A 等细胞因子,则需要设置同型对照,实现阴阳性细胞群的良好区分。 CD4 CD25 (2)在某些特殊情况下,如单核细胞被 PE/Cyanine 7、APC/Cyanine 7 等串联染料标记的抗体染色时,使用同型对照抗体是十分有必要的。单核细胞一般倾向于与 Cyanine7 等染料非特异性结合,使用同型对照抗体有助于获得理想结果。 (3)不清楚抗原表达情况的时候,一定要使用同型对照来界定阴性细胞群。 (4)胞内染色时











