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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
C57BL/6N mouse T cell lymphoma EL-4 cell line
- 保存条件:
Store vial at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
仓鼠
- 应用范围:
Flow
- 抗体英文名:
CD28 Antibody [37.51] (PE-Cy5)
- 抗体名:
CD28 Antibody [37.51] (PE-Cy5)
- 规格:
0.1mg
CD28 抗体 [37.51] (PE-Cy5),CD28 Antibody [37.51] (PE-Cy5),CD28 抗体 [37.51] (PE-Cy5),CD28 Antibody [37.51] (PE-Cy5)
产品名称:CD28 抗体 [37.51] (PE-Cy5)-CD28 Antibody [37.51] (PE-Cy5)
产品货号:PSI-98-718-0.1mg
产品规格:0.1mg
背景资料:CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) on B cells and other antigen presenting cells and plays an important role in the interaction between T cells and B cells. CD28 is a costimulatory receptor involved in many, but not all, T-cell independent immune responses.
保存建议:CD28 抗体 [37.51] (PE-Cy5)-CD28 Antibody [37.51] (PE-Cy5)Store vial at 2-8℃
应用类型:Flow
点击:CD28 抗体 [37.51] (PE-Cy5)-CD28 Antibody [37.51] (PE-Cy5)查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验分钟收集细胞; 5. 将上一步离心得到的细胞沉淀,重悬至扩增培养基中,并进行计数。 1.3 细胞刺激(次日) 扩增培养基成分:RPMI1640 + 10% 血清 + 5ug/ml anti-mouse CD28(克隆号:37.51) + 双抗 1. 将细胞浓度调整至 1-2×10^6/ml(本次实验为 2×10^6/ml,该浓度偏大); 2. 从 4°C 冰箱取出前一天包被的板子,弃掉包被液,无菌PBS洗涤3遍; 3. 每孔加入 500μl 细胞悬液,放置于 37°C 含 5% CO2 细胞
: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation
dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA
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