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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD28
- 亚型:
Hamster IgG
- 形态:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
PE-CY5
- 适应物种:
Mouse
- 宿主:
Hamster
- 应用范围:
FC
- 浓度:
Concentration:0.2 mg/ml
- 靶点:
Clone number:37.51
- 抗体英文名:
CD28 Antibody-PE-CY5;流式抗体
- 抗体名:
CD28 Antibody-PE-CY5;流式抗体
- 规格:
25/100 μg
克隆类型 :Hamster Monoclonal Antibody;Clone number:37.51
货 号 :HT1610189
规 格 :25/100 μg ;
亚 型 :
Hamster IgG
浓 度 :
Concentration:0.2 mg/ml
缓 冲 液 :
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 :
CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.
适用物种 :
Mouse
应用范围 :
FC
应用说明 :
The amount of the reagent is suggested to be used ≤ 1.0 µg /10^6 cells in 100 µl
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
PE-CY5(其他荧光标记请咨询我们)
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文献和实验分钟收集细胞; 5. 将上一步离心得到的细胞沉淀,重悬至扩增培养基中,并进行计数。 1.3 细胞刺激(次日) 扩增培养基成分:RPMI1640 + 10% 血清 + 5ug/ml anti-mouse CD28(克隆号:37.51) + 双抗 1. 将细胞浓度调整至 1-2×10^6/ml(本次实验为 2×10^6/ml,该浓度偏大); 2. 从 4°C 冰箱取出前一天包被的板子,弃掉包被液,无菌PBS洗涤3遍; 3. 每孔加入 500μl 细胞悬液,放置于 37°C 含 5% CO2 细胞
transfer of residual antibody. Vials of Dynabeads® CD3⁄CD28 CTS™ are at negative pressure – liquid must be displaced with air and vice versa. 2) Place one 10 ml vial of Dynabeads® CD3⁄CD28 CTS™ on the MPC-1 for 1 min. 3)
Dual- and Triple-Color Fluorospot
stimulatory agents of choice and anti-CD28 (0.1 μg/ml; seeNote 4), in a final volume of 100–150 μl/well. The number of cells per well is dependent on the stimulating agent used (seeNote 5). b. Incubate the plate overnight at 37°C in a humidified
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