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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD279 (PD-1)
- 级别:
CD279 (PD-1) Antibody
- 目录编号:
CD279 (PD-1) Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
CD279 (PD-1) Antibody
- 抗体英文名:
Cyanine5.5 anti-mouse CD279 (PD-1) Antibody/PerCP
- 抗体名:
CD279 (PD-1) Antibody
- 标记物:
PerCP
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
CD279 (PD-1) Antibody
- 亚型:
CD279 (PD-1) Antibody
- 形态:
CD279 (PD-1) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/ml
- 规格:
25 µg/100 µg
| 规格: | 25 µg | 产品价格: | ¥2200.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥5900.0 |
Description :CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging tothe CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells andthymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyteclonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positiveselection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation isessential for prolonged survival of intratesticular islet allografts.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :PD-1 cDNA followed by PD-1-Ig fusion protein
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5under optimal conditions.
Concentration :0.2 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
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文献和实验1. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
2. Lázár-Molnár E, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2658. (Block)
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706. (FC, IHC, Block)
4. Tobias J, et al. 2020. Front Immunol. 11:895 (FC, ELISA) PubMed
5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations:
1. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
2. Hansel C, et al. 2019. Front Pharmacol. 10:244. PubMed
3. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
4. Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed
5. Utz B, et al. 2020. Sci Rep. 10:18733. PubMed
6. Gozgit JM, et al. 2021. Cancer Cell. :. PubMed
7. Pascual–García M, et al. 2019. Nat Commun. 10:2416. PubMed
8. Haikala HM, et al. 2019. Nat Commun. 10:620. PubMed
9. Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed
10. Wu L, et al. 2020. Cell Rep. 33:108327. PubMed
11. Hekim C, et al. 2017. Cancer Immunol Res. 5:157. PubMed
12. Barbet G, et al. 2018. Immunity. 48:584. PubMed
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