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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD319
- 级别:
CD319 (CRACC) Antibody
- 目录编号:
CD319 (CRACC) Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
CD319 (CRACC) Antibody
- 抗体英文名:
Dazzle™ 594 anti-human CD319 (CRACC) Antibody-PE
- 抗体名:
CD319 (CRACC) Antibody
- 标记物:
PE
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
CD319 (CRACC) Antibody
- 亚型:
CD319 (CRACC) Antibody
- 形态:
CD319 (CRACC) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
CD319 (CRACC) Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥2700.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥6300.0 |
Description :CD319 is a single-pass type l transmembrane glycoprotein, expressed on NK cells, subsets ofmature dendritic cells, activated B cells, and cytotoxic lymphocytes, but not in promyelocytic, Bor T cell lines. Expression is highest in the spleen, lymph nodes, and peripheral bloodleukocytes, and lowest in bone marrow. Additionally, it is expressed in the small intestine,stomach, appendix, lung, and trachea. CD319 is tyrosine phosphorylated in activated NK cellsand is associated with 19 and 39 kD proteins. CD319 has homology with the CD2 family ofreceptors within the Ig superfamily. Some of the CD2 members stimulate cytotoxicity throughthe CD319 associated protein.
Verified Reactivity :Human
Reported Reactivity :Cynomolgus
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :CRACC-Human IgG1 fusion protein
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Bouchon A, et al. 2001. J. Immunol. 167:551721.
2. Tassi I and Colonna M. 2005. J. Immunol. 175:79968002.
3. Veillette A. 2006. Immunol. Rev. 214:2234.
度的功能细胞。二、人外周血中 CD4 T 细胞分选实例1.产品信息: MojoSort™ Human CD4 T Cell Isolation Kit(BioLegend, Cat#480009),阴性分选试剂盒2.检测仪器型号:BD LSRⅡ3.样本:正常人外周血4.分选前后检测相关产品:PE-CD4(Biolegend, Cat# 317410)5.实验过程(1)采血之后采用达优™ 人淋巴细胞分离液分离人外周血单个核细胞。分离之后能见到明显的白膜层。(达优,Cat#DKW-KLSH-0100
5.5, APC等。 常见荧光素波长分布示意图 同型对照的选择: 流式细胞实验和其他抗体相关实验有点不同的就是需要选择同型对照。同型对照,是用于消除由于抗体非特异性结合到细胞表面而产生的背景染色,相当于实验的阴性对照。同型对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型
非特异性结合到细胞表面而产生的背景染色,相当于实验的阴性对照。同型对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型对照选择PE标记的小鼠的IgG2a。 流式抗体使用注意事项: 1. 建议实验细胞的数量和抗体的比例要适当。细胞过量或抗体过量都可能使实验结果受影响,因此需要优化试验条件。注:不同的流式技术对抗体的需求量有较大差异,例如传统流式细胞术(采用鞘液流系统)需要1×106个细胞为起始上样量,抗体用量为10 μl










