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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD196 (CCR6)
- 级别:
CD196 (CCR6) Antibody
- 目录编号:
CD196 (CCR6) Antibody
- 克隆性:
单克隆
- 抗原来源:
亚美尼亚仓鼠
- 保质期:
CD196 (CCR6) Antibody
- 抗体英文名:
Cyanine5.5 anti-mouse CD196 (CCR6) Antibody/PerCP
- 抗体名:
CD196 (CCR6) Antibody
- 标记物:
PerCP
- 宿主:
亚美尼亚仓鼠
- 适应物种:
小鼠
- 免疫原:
CD196 (CCR6) Antibody
- 亚型:
CD196 (CCR6) Antibody
- 形态:
CD196 (CCR6) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/ml
- 规格:
25 µg/100 µg
| 规格: | 25 µg | 产品价格: | ¥3200.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥7000.0 |
Description :CCR6 is a G-protein linked chemokine receptor which binds the chemokine CCL20/MIP-3a. It isexpressed on B lymphocytes and certain subsets of dendritic cells and T cells. CCR6 isreported to be involved in mucosal immune response and lymphocyte migration andhomeostasis.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Armenian Hamster
Immunogen :N-terminal peptide (aa. 2-38) of mouse CCR6 fused with GST
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5under optimal conditions.
Concentration :0.2 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
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文献和实验1. Zhan CS, et al. 2020. FASEB J. 14006:34. PubMed
2. Ghilas S, et al. 2021. iScience. 24:103059. PubMed
3. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
4. Lind L, et al. 2021. J Neurovirol. 27:145. PubMed
5. Pardo E, et al. 2017. PLoS One. 12(6):e0177472. PubMed
6. Goode D, et al. 2014. PLoS Pathog. 10:1004567. PubMed
有好几个同仁发消息问CD31染色,刚工作比较忙,今天分享一下我做的是免疫荧光,也试做过免疫组化,都可以做出来抗体:小鼠抗CD31单克隆抗体 英国Abcam公司(CD31抗体说明书里面有说多聚甲醛会掩盖抗体,所以推荐速冻切片,也尝试过速冻切片,不使用多聚甲醛,但是这个技术个人掌握不好,所以还是用了多聚甲醛固定)二抗: 山羊抗小鼠488单克隆抗体 美国CST公司 特别说明:我是从同学那里得到的经验,可能绿光稍微好做些,之前做红光
,而流式细胞仪只能做4个通道,首先将指标归成2类,再将样本分成2份,分别检测。比如需要同时检测CD3、CD4、CD8、IL-4和IFN-gamma,那么将样本分成两份,第1份加CD3、CD4、CD8和IL-4,而第2份加CD3、CD4、CD8和IFN-gamma。 二、同型对照(Isotypy Control) 1、为什么要用同型对照? 同型对照是使用与一抗相同种属来源、相同亚型、相同剂量和相同的免疫球蛋白及亚型的免疫球蛋白,用于消除由于抗体非特异性与细胞
/Cytoperm液、Perm/Wash液和FACS Lysing Solution,均购自BD Pharmingen。(4)抗体细胞表面标记抗体:抗人CD3-APC,CD8-PercP,CD8-CYC,CD4-CYC。抗小鼠CD4-CYC、CD8PE。细胞内因子抗体:抗人IL-4-PE,IFN-γ-FITC。抗鼠IL-4-PE,IFN-γ-FITC。以上抗体均购自BD Pharmingen。2、实验方法(1)外周血单个核细胞的分离:取肝素抗凝正常人或者Balb/c小鼠外周血1.5ml。在试管内加入淋巴










