Description :CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25kD type I transmembrane protein and a member of B7 family within the immunoglobulinreceptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset ofmacrophages as well as a few transformed cell lines. CD273 has been reported to bestimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engagingthe PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and tomediate T cell activation through such non-PD1 mediated interactions.
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.