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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD273 (B7-DC, PD-L2)
- 级别:
CD273 (B7-DC, PD-L2) Antibody
- 目录编号:
CD273 (B7-DC, PD-L2) Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
CD273 (B7-DC, PD-L2) Antibody
- 抗体英文名:
Dazzle™ 594 anti-human CD273 (B7-DC, PD-L2) Antibody-PE
- 抗体名:
CD273 (B7-DC, PD-L2) Antibody
- 标记物:
PE
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
CD273 (B7-DC, PD-L2) Antibody
- 亚型:
CD273 (B7-DC, PD-L2) Antibody
- 形态:
CD273 (B7-DC, PD-L2) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
CD273 (B7-DC, PD-L2) Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥2900.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥6500.0 |
Description :CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25kD type I transmembrane protein and a member of B7 family within the immunoglobulinreceptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset ofmacrophages as well as a few transformed cell lines. CD273 has been reported to bestimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engagingthe PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and tomediate T cell activation through such non-PD1 mediated interactions.
Verified Reactivity :Human
Reported Reactivity :African Green, Baboon, Cynomolgus, Rhesus
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Human PD-L2 cDNA
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Brown JA, et al. 2003. J. Immunol. 170:1257
2. Rodig N, et al. 2003. Eur. J. Immunol. 33:3117
Product Citations:
1. Martín–Gayo E, et al. 2020. Cell Rep. 30:984. PubMed
机体免疫应答过程的复杂性和严格的调控性,是由多种免疫细胞和免疫分子共同参与完成的。而T淋巴细胞的活化,在免疫应答中扮演着相当重要的角色。研究表明,诱导T细胞的活化与增殖需要两种信号:一种是 TCR/CD3 与抗原提呈细胞(APCs)表面特异的 MHCⅡ 抗原肽复合物结合产生的特异性抗原刺激信号;另一种是非特异性协同刺激信号,由 APCs 表面的协同刺激分子和 T 细胞的相应受体相互作用后产生,其中 CD28/B7 是最为重要的协同刺激分子,能增加 IL-2 的产生,加速T细胞增殖,阻止细胞进入
度的功能细胞。二、人外周血中 CD4 T 细胞分选实例1.产品信息: MojoSort™ Human CD4 T Cell Isolation Kit(BioLegend, Cat#480009),阴性分选试剂盒2.检测仪器型号:BD LSRⅡ3.样本:正常人外周血4.分选前后检测相关产品:PE-CD4(Biolegend, Cat# 317410)5.实验过程(1)采血之后采用达优™ 人淋巴细胞分离液分离人外周血单个核细胞。分离之后能见到明显的白膜层。(达优,Cat#DKW-KLSH-0100
Nature Cancer I 生物标志物多参数评估mTLS预测实体瘤免疫治疗效果
了 TLS 中 B 细胞通过分泌抗体和细胞因子对抗 PD-1/抗 PD-L1 的反应起关键作用。DC 通过调节抗原加工和呈递来调节 T 细胞功能。B 细胞的功能依赖于 TLS 成熟度,可能是促肿瘤或抗肿瘤。在成熟 TLS 中,B 细胞和 DC 可以通过向 CD8+ 细胞提供肿瘤抗体来更好地训练 CD8+ T 细胞。和明场染色方法相比,Opal 多重免疫荧光染色同时检测更多指标,更可靠地评估 TLS 的成熟度状态。 CD3:T 细胞; CD8: 杀伤性 T 细胞;PD-L-1: 程序性死亡配体











