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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD51/61
- 级别：
CD51/61 Antibody
- 目录编号：
CD51/61 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
CD51/61 Antibody
- 抗体英文名：
anti-human CD51/61 Antibody-FITC
- 抗体名：
CD51/61 Antibody
- 标记物：
FITC
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
CD51/61 Antibody
- 亚型：
CD51/61 Antibody
- 形态：
CD51/61 Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
CD51/61 Antibody
- 规格：
25 tests/100 tests
| 规格： | 25 tests | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100 tests | 产品价格： | ￥3200.0 |
Description ：CD51/CD61 is an integrin complex known as α β . It is expressed at high levels on osteoclasts,endothelial cells, and melanoma cells and at low levels on platelets and macrophages. CD51 isa heterodimer composed of disulfide-linked 125 kD and 24 kD proteins. CD61 is also amember of the integrin family known as gpIIIa or β integrin. It is a 110 kD common β subunit ofCD51/CD61 or CD41/CD61 complex. CD51/CD61, also known as the vitronectin receptor,mediates the binding of platelets to immobilized vitronectin without prior activation. Otherligands include RGD-containing proteins such as fibrinogen, fibronectin, von Willebrand factor(vWf), laminin, thrombospondin and the neural adhesion molecule L1. CD51/CD61 alsomediates cell-cell adhesion via interaction with CD31. CD51/CD61 acts as an activationindependent receptor for platelet attachment and spreading on vitronectin and other RGDcontaining proteins, including matrix components. The 23C6 antibody has been reported to beuseful for blocking studies.
Verified Reactivity ：Human
Reported Reactivity ：African Green, Chicken, Cynomolgus, Rabbit
Antibody Type ：Monoclonal
Host Species ：Mouse
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation ：The antibody was purified by affinity chromatography, and conjugated with FITC under optimalconditions.
Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser ：Blue Laser (488 nm)
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文献和实验1. Knapp WB, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
3. Horton M, et al. 1991. Exp. Cell Res. 195:368.
4. Takahashi R, et al. 1999. Blood 93:1951. (Block)
5. Davies J, et al. 1989. J. Cell Biol. 109:1817. (IF, IHC, IP)
6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709. (FC, Block)
7. Barau A, et al. 2010. J. Ultrasound Med. 29:173. PubMed
Product Citations：
1. Wu W, et al. 2013. J Lipid Res. 54:936. PubMed
当FITC在碱性溶液中与抗体蛋白反应时，主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合，形成FITC-蛋白质结合物，即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基，一般最多能结合15～20个，一个IgG分子可结合2～8个分子的FITC，其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体，也可以根据条件采用Chadwick等标记法或Clark
ml三蒸水中即成； 方法与步骤： 根据Marshall氏法高效价的抗人球蛋白兔免疫血清，分离球蛋白。 1. 用0.15 mol/L NaCl的盐水及0.15 mol/L pH9.0的NaHCO3-Na2CO3缓冲液稀释使每毫升内含抗体10mg，缓冲液为总量的10%； 2. 将以上溶液降温至4℃，按蛋白：荧光素=50—80mg：1mg的比例加入异硫氰酸荧光素，在0—4℃下电磁搅拌12—14h； 3.用半饱和硫酸铵将标记球蛋白
抗体二抗上连接有荧光染料。直接标记染色背景低，实验程序少尽量减少实验工序和过程，以保证实验的真实和准确性。因此在条件允许的范围内，建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择： 如果实验中检测单一指标：不同荧光标记在不同的仪器上强度不同。以某仪器为例：PE ＞APC ＞PE-Cy5 ＞PerCP ＞FITC ＞PerCP-Cy5.5，通常来说，PE最强，适用于弱表达抗原。FITC强度较弱，适用于强表达抗原，使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
