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- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse CEACAM5 Protein (His & AVI Tag), Biotinylated
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20 µg/100 µg
| 规格: | 20 µg | 产品价格: | ¥3870.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥8940.0 |
蛋白名称:Mouse CEACAM5 Protein (His & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the Mouse CEACAM5 (Q3UKK2-1) (Gln35-Glu947) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性:见说明书
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Gln 35
蛋白分子量:The recombinant Mouse CEACAM5 consists of 939 amino acids and predicts a molecular mass of 105.88 kDa. It migrates as an approximately 124.9 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:Q3UKK2-1
蛋白氨基酸序列:Gln35-Glu947
蛋白标签:C-His-AVI
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文献和实验Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
RNAse A Treatment of Mouse Cells
of the quality of the used RNAse A. In our lab, we used RNAse A treatment of mouse cells (see comment 1 ) to demonstrate that the enrichment in HP1 (Heterochromatin Protein 1) proteins at pericentric heterochromatin depends on the presence of an RNA component
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