Description :CD314 is a homodimeric C-type lectin-like protein also known as NKG2D. It is expressed on NKcells, CD8 T cells, γ/δ T cells, and in vitro induced LAK cells. Several molecules have beenidentified as the ligands for NKG2D, including MHC class-I chain-related protein A (MICA),MICB, and UL16-binding proteins (ULBPs). NKG2D has no intrinsic signaling capacity, butattains this by non-covalent association with DAP10 or DAP12 adaptors. In addition to being aprimary activation receptor on NK cells, NKG2D is also a costimulatory receptor for TCRmediated T cell proliferation and cytokine production. The interaction of NKG2D with its ligandsplays a role in the immune surveillance against pathogen and tumor cells, and in thepathogenesis of autoimmune diseases.
Formulation :0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxinlevel is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation :The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration :The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentrationand expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™solution contains no preservative; handle under aseptic conditions.
Application :FC - Quality tested、IP, FA - Reported in the literature, not verified in house
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cellsin 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated foroptimal performance for each application.