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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD43 Activation
- 级别：
CD43 Activation-Associated Glycoform Antibody
- 目录编号：
CD43 Activation-Associated Glycoform Antibody
- 克隆性：
单克隆
- 抗原来源：
大鼠
- 保质期：
CD43 Activation-Associated Glycoform Antibody
- 抗体英文名：
Cyanine7 anti-mouse CD43 Activation-Associated Glycoform Antibody-PE
- 抗体名：
CD43 Activation-Associated Glycoform Antibody
- 标记物：
PE
- 宿主：
大鼠
- 适应物种：
小鼠
- 免疫原：
CD43 Activation-Associated Glycoform Antibody
- 亚型：
CD43 Activation-Associated Glycoform Antibody
- 形态：
CD43 Activation-Associated Glycoform Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.2 mg/ml
- 规格：
25 µg/100 µg
| 规格： | 25 µg | 产品价格： | ￥2300.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥5200.0 |
Description ：CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115,is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation andsialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have beenidentified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including Tlymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5 B cells. It is not presenton resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activationassociated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It hasbeen reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesionand anti-adhesion, as well as costimulation of T cell activation and survival, and induction ofapoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with theactivation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared withdesialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells andmemory T cells.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Rat
Immunogen ：Mouse WEHI 274.3 myeloid tumor cells
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 underoptimal conditions.
Concentration ：0.2 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10 cells in100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser ：Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB)
2. Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB)
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. van der Most RG, et al. 2003. Intl. Immunol. 15:119.
5. Chu VT, et al. 2007. J. Immunol. 179:5947.
6. Lang A, et al. 2008. J. Immunol. 180:4848. PubMed
7. Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed
Product Citations：
1. Myers LM, et al. 2019. Nat Commun. 10:794. PubMed
2. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
3. Ferreira CP, et al. 2017. Front Immunol. 1.229861111. PubMed
4. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
5. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
6. Stephens R, Langhorne J 2010. PLoS One. 6:e1001208. PubMed
7. Knudsen M, et al. 2014. J Virol. 88:12438. PubMed
8. Ersching J, et al. 2016. PLoS Pathog. 12: 1005698. PubMed
9. Martin P, et al. 2015. Gut. 64: 1961 - 1971. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低，实验程序少尽量减少实验工序和过程，以保证实验的真实和准确性。因此在条件允许的范围内，建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择： 如果实验中检测单一指标：不同荧光标记在不同的仪器上强度不同。以某仪器为例：PE ＞APC ＞PE-Cy5 ＞PerCP ＞FITC ＞PerCP-Cy5.5，通常来说，PE最强，适用于弱表达抗原。FITC强度较弱，适用于强表达抗原，使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
条带。 五、 二抗的选择 二抗应选用与使用的一抗相同的物种来源，例如：如果你的一抗是小鼠的单克隆抗体，二抗则选抗小鼠的二抗anti-mouse secondary。建议检查二抗说明书确保该抗体适用于你的检测应用， 二抗一般连接荧光素FITC 或发光团。 六、双重染色抗体的选择 用未偶联一抗进行细胞培养物或组织切片的双重免疫染色要求一抗来源于不同物种并且二抗分别识别其中之一，二抗说明书应描述其与其它物种来源的免疫球蛋有否有交叉吸附。 七、荧光和化学发光标记 连接于二抗的标记
，比如WB、IHC等，都是通过二抗类的试剂标记达到结果呈现的目的。但是基于仪器分析的一些实验，可能就会使用到直接标记的一抗，比如流式实验。那么需要了解到自己将要使用的仪器能检测到的荧光范围，针对不通的参数要求选择对应的标记物。在免疫荧光双标实验中，需要选配不同的荧光标记物。 2. 抗体种属来源的选择 有人认为单抗比多抗好，其实这并不是一个严谨的观点，只能说在可能性上单抗的特异性更好一些，而多抗的亲和力会优于单抗，并且特异性并不一定就逊于单抗，主要看抗原的设计水平。目前商品化抗体里
