Ultra-LEAF™ Purified 抗人 TSLP 抗

Ultra-LEAF™ Purified anti-human TSLP Antibody详细如下：

Description ：Thymic Stromal Lymphopoietin (TSLP) is a hemopoietic protein that is expressed in the heart,liver and prostate. TSLP overlaps biological activities with IL-7 and binds with the heterodimericreceptor complex consisting of the IL-7R alpha chain (IL-7Rα) and the TSLP-specific chain(TSLPR). Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinasesother than the JAKs for activation. TSLP prohibited apoptosis and stimulated growth of thehuman acute myeloid leukemia (AML)-derived cell line MUTZ3. It induces the release of T cellattracting chemokines TARC and MDC from monocytes and activates CD11c(+) dendritic cells(DCs). TSLP activated DCs primed naïve T cells to produce the proallergic cytokines (IL-4, IL5, IL-13, TNFα) while down-regulating IL-10 and IFN-γ suggesting a role in initiating allergicinflammation. Recent studies revealed that expression of TSLP has a direct link to thepathogenesis of allergic inflammation such as atopic dermatitis and asthma via activatingdendritic cells and mast cells which trigger inflammatory Th2 response characterized by highTNF-alpha and little IL-10 production.

Verified Reactivity ：Human

Antibody Type ：Monoclonal

Host Species ：Mouse

Immunogen ：Recombinant human TSLP

Purity ：The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Formulation ：0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxinlevel is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Concentration ：The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentrationand expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™solution contains no preservative; handle under aseptic conditions.

Application ：ELISA Capture - Quality tested、Neut - Reported in the literature, not verified in house

Recommended Usage ：Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detectionantibody, a concentration range of 1.0 - 4.0 µg/mL is recommended. It is recommended that thereagent be titrated for optimal performance for each application. To obtain a linear standard curve,serial dilutions of TSLP recombinant protein ranging from 200 to 3.1 ng/mL are recommended foreach ELISA plate.