- ￥2500 - 5900
- 齐源
- 151209
- 国产/进口
- 2025年07月15日
- ICFC
- 大鼠
- 人、小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
Ki-67
- 级别：
mouse/human Ki-67 Antibody
- 目录编号：
mouse/human Ki-67 Antibody
- 克隆性：
单克隆
- 抗原来源：
大鼠
- 保质期：
mouse/human Ki-67 Antibody
- 抗体英文名：
anti-mouse/human Ki-67 Antibody-PE
- 抗体名：
mouse/human Ki-67 Antibody
- 标记物：
PE
- 宿主：
大鼠
- 适应物种：
人、小鼠
- 免疫原：
mouse/human Ki-67 Antibody
- 亚型：
mouse/human Ki-67 Antibody
- 形态：
mouse/human Ki-67 Antibody
- 应用范围：
ICFC
- 保存条件：
2-8℃
- 浓度：
0.2 mg/ml
- 规格：
25 µg/100 µg
| 规格： | 25 µg | 产品价格： | ￥2500.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥5900.0 |
Description ：The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which wasgenerated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 proteinplays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable.Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic markerin various tumors.
Verified Reactivity ：Mouse, Human
Antibody Type ：Monoclonal
Host Species ：Rat
Immunogen ：E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinantprotein.
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography and conjugated with PE under optimalconditions.
Concentration ：0.2 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：ICFC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by intracellular immunofluorescent staining withflow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µgper million cells in 100 µl volume. It is recommended that the reagent be titrated for optimalperformance for each application.
Excitation Laser ：Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
2. Lin J, et al. 2021. Front Immunol. 12:796260. PubMed
3. Low MSY, et al. 2019. Cell Reports. 29(9):2634-2645.e5. PubMed
4. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
5. Hasmim M, et al. 2022. Front Immunol. 13:982821. PubMed
6. Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed
7. Zotos D, et al. 2021. Nat Commun. 12:7160. PubMed
8. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
9. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
10. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
11. Wang H, et al. 2019. Stem Cells Int. 2019:4686132. PubMed
12. Shan L, et al. 2017. Immunity. 47:766. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低，实验程序少尽量减少实验工序和过程，以保证实验的真实和准确性。因此在条件允许的范围内，建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择： 如果实验中检测单一指标：不同荧光标记在不同的仪器上强度不同。以某仪器为例：PE ＞APC ＞PE-Cy5 ＞PerCP ＞FITC ＞PerCP-Cy5.5，通常来说，PE最强，适用于弱表达抗原。FITC强度较弱，适用于强表达抗原，使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
sunchangzheng 我打算用此抗体通过流式检测细胞表面P糖蛋白的表达量差异。 说明书中说是多抗，这种抗体有单抗的吧？ 用于FCM： 1：20-100，这是说用于流式的稀释倍数，是不是稀释倍数越小越好呢？ 同型对照抗体是不是必须要做的？如何选择阴型同型对照抗体呢！ 我用的抗体是FITC标记的P-糖蛋白抗体 谢谢交流与帮助！ freecell 抗体的稀释倍数是需要优化
T-cell dependent antigen specific in situ staining (Pe)
Double staining for Ag and Ki-67, TUNEL or BCL-6. Cut frozen tissue sections (spleen, lymph nodes) in a cryostat at ~ 4µm, on plain glass slides (Adhesive -coated slides are sometimes too hydrophobic, but fine
