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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD43 Activation
- 级别：
CD43 Activation-Associated Glycoform Antibody
- 目录编号：
CD43 Activation-Associated Glycoform Antibody
- 克隆性：
单克隆
- 抗原来源：
大鼠
- 保质期：
CD43 Activation-Associated Glycoform Antibody
- 抗体英文名：
Alexa Fluor® 488 anti-mouse CD43 Activation-Associated Glycoform Antibody
- 抗体名：
CD43 Activation-Associated Glycoform Antibody
- 标记物：
CD43 Activation-Associated Glycoform Antibody
- 宿主：
大鼠
- 适应物种：
小鼠
- 免疫原：
CD43 Activation-Associated Glycoform Antibody
- 亚型：
CD43 Activation-Associated Glycoform Antibody
- 形态：
CD43 Activation-Associated Glycoform Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
100 µg
Description ：CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115,is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation andsialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have beenidentified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including Tlymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5 B cells. It is not presenton resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activationassociated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It hasbeen reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesionand anti-adhesion, as well as costimulation of T cell activation and survival, and induction ofapoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with theactivation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared withdesialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells andmemory T cells.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Rat
Immunogen ：Mouse WEHI 274.3 myeloid tumor cells
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation ：The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488under optimal conditions.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10 cells in100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Excitation Laser ：Blue Laser (488 nm)
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文献和实验1. Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB)
2. Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB)
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. van der Most RG, et al. 2003. Intl. Immunol. 15:119.
5. Chu VT, et al. 2007. J. Immunol. 179:5947.
6. Lang A, et al. 2008. J. Immunol. 180:4848. PubMed
7. Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed
Product Citations：
1. Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
技术原理与方法｜超级色差校正物镜 PLAPON60XOSC 在脑组织四重免疫荧光分析中的应用
种类型的荧光抗体在单个标本中准确展示荧光标记分子的共定位情况。 PLAPON60XOSC 和 UPLSAPO60XO 的性能比较 2. 超级色差校正物镜的应用-脑组织的四重免疫荧光 图 1. 脑组织的四重免疫荧光。 VIAAT 染色（Alexa Fluor405，蓝色）；CB1 染色（Alexa Fluor488，绿色）；VGluT3 染色（Cy3，红色）；DGLα染色（Alexa Fluor647，白色）。 大麻素合成酶 DGLα（白色）和大麻素受体 CB1（绿色）聚集在小鼠
常用抗体标记荧光染料的选择 随着免疫荧光技术的不断发展，荧光染料及其标记的抗体偶联物也被广泛的应用于生物学实验中。目前，市场上抗体及蛋白标记的荧光染料主要有CFTM系列(BIOTIUM, USA); Alexa Fluor®系列 (Life technology, USA); DyLight系列;Cy系列; IR Dye系列等等。使用最多的为AlexaFluor®系列和CFTM系列。CFTM系列染料的核心对比AlexaFluor®系列具有以下几点优势：1. 新型罗丹明核心罗丹明染料以优异
，Alexa Fluor 488）和γH2AX（红色，Alexa Fluor 594）。通道合并图像显示 CDH4 和γH2AX 的共定位。
