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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
SH2D1A (SAP)
- 级别:
SH2D1A (SAP) Antibody
- 目录编号:
SH2D1A (SAP) Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
SH2D1A (SAP) Antibody
- 抗体英文名:
Purified anti-SH2D1A (SAP) Antibody
- 抗体名:
SH2D1A (SAP) Antibody
- 标记物:
SH2D1A (SAP) Antibody
- 宿主:
大鼠
- 适应物种:
人、小鼠
- 免疫原:
SH2D1A (SAP) Antibody
- 亚型:
SH2D1A (SAP) Antibody
- 形态:
SH2D1A (SAP) Antibody
- 应用范围:
WB
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
100 µg
Description :SH2D1A, also known as SAP, is a small adaptor protein which plays a major role in thebidirectional stimulation of T and B cells. SH2D1A is predominantly expressed in T cells and NKcells, and functions to regulate signal transduction pathways downstream of the SLAM family ofsurface receptors. The SH2D1A gene is frequently mutated in patients with X-linkedlymphoproliferative disease. Malfunction of the SLAM-SAP pathway results in autoimmunediseases, tumors of the lymphoid tissues and inadequate responses to infectious agents.
Verified Reactivity :Human, Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :Full length purified human SH2D1A.
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C.
Application :WB - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by Western blotting. For Western blotting, thesuggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titratedfor optimal performance for each application.
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文献和实验两者所用的显色系统也不同。SP法与SAP法的其他染色步骤同SABC法。 Envision TM SVStemS 该法是近几年在国内推广使用的一种方法,它的原理是将多个抗鼠和抗兔IgG分子与辣根过氧化物酶结合形成聚合物。以代替传统方法的二抗和三抗,直接与特异性第一抗体结合,从而放大了抗原抗体结合的信号,使检测变得简单而且敏感性增加。经DAB显色即可完成染色,其他染色步骤与SABC法相同。 Envision TM SVStemS原理示意图
c.两只山羊,每只均经过四次免疫 d.1,000~1,200ml抗血清 e.Protein G亲和纯化100ml抗血清 f.ELISA测试 A.2~5ml免疫前血清 B.ELISA结果 C.100ml抗血清中经Purified G纯化的抗体 C.≈1000ml抗血清 E.5mg未偶联多肽,MS和HPLC报告
培养箱中培养。 2、实验结果 图:1×10^6/孔细胞,5μg anti-mouse CD3与CD28刺激3天后细胞形成明显克隆团 CD3/CD28 Streptamer® 多聚体法 1、实验原理 使用低亲和力的抗 CD3 和抗CD28的抗体 Fab 片段(非传统全长抗体),结合上 Twin-Strep-tag 亲和标签,形成 CD3 Fab-Strep 和 CD28 Fab-Strep。再结合可溶性蛋白多聚体 Strep-Tactin®,在亲和力作用下,即可与 TCR 和 CD28 共刺激










