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文献和实验Assessing Matrix Metalloproteinase Expression and Activity in Hepatocellular Carcinomas
tissue inhibitors of matrix metalloproteinase (TIMPs). MMPs are involved in situations that require extracellular matrix remodeling, including wound healing, development, inflammation, fibrosis angiogenesis, and tumor invasion (2 –
Monoclonal Antibody Affinity Chromatography
. These criteria are often best fulfilled for protein targets by antibody ligands. Monoclonal antibodies are the ligands of choice for most purposes, especially when a protein of unknown properties is being characterized. Monoclonal antibodies fulfill
Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography
to produce monoclonal antibodies (MAbs) were developed, purification of antigens has been facilitated with MAbs coupled to Sepharose 4B (Pharmacia Biotech Inc., Piscataway, NJ). MAb reagents coupled to this support matrix require reduced time to purify
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