Human Secreted Phosphoprotein

人工转录因子的部件――人类锌指结构

of VEGF protein secreted from HEK 293 cells that had been transiently transfected with the corresponding ZFPs; measured with an ELISA assay kit (Chemicon, Temecula, CA). 'Vector' indicates a control vector that encode no ZFPs. An 'irrelevant ZFP

Intracellular Immunofluorescent Staining for Flow Cytometry

for detection of IL-6-producing cells by human LPS-activated monocytes is 6 hours, whereas IL-10 detection needs at least 24 hours stimulation. In contrast to detection of secreted cytokines by ELISA, for detection of intracellular cytokines

Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays

phosphatase‐conjugated secondary antibody Substrate 104 (or other alkaline phosphatase substrate; Sigma‐Aldrich) ELISA substrate