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- Biomatik
- CAU31496
- 2025年07月15日
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文献和实验. Transfer 5 µl of the PCR product to another PCR tube. Bring the volume to 25 µl with a newly prepared PCR mix containing [{alpha}-32P]-dCTP (10 µCi/µl, specific activity 3000 Ci/mmol) and fresh dNTPs. The amount of [{alpha}-32P]dCTP
of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins. A polyclonal antibody was raised against purified ST-IV. A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti
Antigen Design Sera Purification Tech Sheet
to the References1,2. Peptide Selection and Design The first step in the process is the selection of the appropriate peptide sequence. At this step the ultimate use for the antibody must be considered. If the antibody is needed to probe a specific protein
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