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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD3
- 级别:
CD3 Antibody
- 目录编号:
CD3 Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
CD3 Antibody
- 抗体英文名:
TotalSeq™-B0182 anti-mouse CD3 Antibody
- 抗体名:
CD3 Antibody
- 标记物:
CD3 Antibody
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
CD3 Antibody
- 亚型:
CD3 Antibody
- 形态:
CD3 Antibody
- 应用范围:
PG
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
10 ug
TotalSeq™-B0182 anti-mouse CD3 Antibody详细如下:
Description :CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells,NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composedof CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains.CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognitionby binding the peptide/MHC antigen complex
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :γδTCR-positive T-T hybridoma D1
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation :The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer underoptimal conditions.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application :PG - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-B antibodies arecompatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.
Description :CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells,NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composedof CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains.CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognitionby binding the peptide/MHC antigen complex
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :γδTCR-positive T-T hybridoma D1
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation :The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer underoptimal conditions.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application :PG - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-B antibodies arecompatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.
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文献和实验该产品被引用文献
Application References(PubMed link indicatesBioLegend citation):
1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
文献支持
TotalSeq™-B0182 抗小鼠 CD3 抗体
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