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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD3
- 级别:
CD3 Antibody
- 目录编号:
CD3 Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
CD3 Antibody
- 抗体英文名:
anti-mouse CD3 Antibody-APC
- 抗体名:
CD3 Antibody
- 标记物:
APC
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
CD3 Antibody
- 亚型:
CD3 Antibody
- 形态:
CD3 Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/ml
- 规格:
25 ug/100 ug
| 规格: | 25 ug | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 100 ug | 产品价格: | ¥1400.0 |
Description :CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells,NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composedof CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains.CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognitionby binding the peptide/MHC antigen complex
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :γδTCR-positive T-T hybridoma D1
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with APC under optimalconditions.
Concentration :0.2 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
Excitation Laser :Red Laser (633 nm)
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文献和实验1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations:
1. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
2. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
3. Calabrese DR, et al. 2020. J Clin Invest. . PubMed
4. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
5. Liu J, et al. 2019. Immunity. 50:600. PubMed
6. Rivas MA, et al. 2021. Nat Immunol. 22:240. PubMed
7. Xiang G, et al. 2022. Cell Death Dis. 13:451. PubMed
8. Pani F, et al. 2021. Endocrinology. 162:. PubMed
9. Naito Y, et al. 2019. American Journal of Physiology-Renal Physiology. 318(1):F238-F247.PubMed
10. Yang H, et al. 2019. Mol Metab. 23:24. PubMed
11. Gabriely G, et al. 2021. iScience. 24:103347. PubMed
12. Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
1 ml 2.4 中准备好的 Biotin Elution Buffer,让液流完全进入胶体后孵育 2 min,加入 9 ml 的 Biotin Elution Buffer; 1.13 对于从全血中分选目的细胞,再加入 10 ml 的 Biotin Elution Buffer; 1.14 300 g 转速离心 10 分钟,收集洗脱目的细胞。 流程图: 2. 实验数据 以下流式图为从 7.5 ml 全血中,选出 CD3 + T 细胞的数据。选后细胞以 CD3 -APC (OKT
: 由此步骤开始,流出的溶液包含目标细胞,请使用新的试剂管收集!首先,加入 1 ml 的 biotin 洗脱液,孵育 2 min。其次,加入 10 ml biotin 洗脱液,洗脱目标细胞。d) 再加入 10 ml biotin 洗脱液,洗下剩余的目标细胞。最后两步骤所收集的液体含分选出的目标细胞。(4) 结果分析以下流式图为从 7.5 ml 全血中,选出 CD3+ T 细胞的数据。选后细胞以 CD3-APC(OKT-3) 及 CD45-PE(HI30,白细胞标志) 抗体染色后,进行流式分析,死细胞











